Open the output file on a dark background. Just do it. Half the transparency issues I have seen in production were invisible on white backgrounds. The alpha channel looked fine until someone dropped the image onto a dark mode UI, and suddenly there was a white halo around every edge.
This single habit — flipping the background from light to dark — has caught more bad exports than every other check combined. It takes five seconds and has saved me from redoing entire batches. RFC 4648 — the base64 alphabet and padding rules documents why background handling matters, and base64 file decoder is what I use when I need to compare quickly.
I also zoom to 100% and inspect one edge. Zoomed out, compression artifacts hide in gradients; zoomed in, you can see whether the file actually survived the conversion intact. Ten seconds of inspection prevents a support ticket a week later.
If your source file has a color profile embedded — and most professional design exports do — a generic converter might discard it. The result looks fine on your screen but prints with shifted colors. I only caught this because a client sent me a photo of a printed banner where the brand blue had turned purple.
Now I make sure the converter preserves embedded ICC profiles, and I check the output against Wikipedia — Base64: every 3 input bytes become 4 output characters expectations for color handling. It is one of those things you do not think about until it burns you. Then you never forget.
The same applies to metadata. Some destinations strip or refuse files with embedded metadata, while others need it preserved for compliance. Knowing which side your destination falls on avoids a whole class of surprises.
I open the output in two environments: the one it was built for, and the opposite one. If it was built for a light-background webpage, I also check it on a dark background. If it was built for print, I also check it on a phone screen at 2x zoom. This catches 90% of the problems that slip past automated validation.
It adds maybe ninety seconds to my workflow. Compared to the hours of rework it prevents, it is the cheapest insurance I have. I run this same pass whether I converted one file or one thousand.
Cross-checking against the destination requirements in RFC 2045 — MIME base64, the original email use case keeps me honest. The spec describes the minimum; real-world rendering demands more. Testing both extremes is how I find the gap before a customer does.
At the end of the day, the goal is an output you do not have to worry about. If a single step here saves you one redo, it was worth the read. I keep MDN btoa() and atob() reference — the Latin-1 limitation bookmarked for the days I doubt myself, and I run my checks on every export before it ships.